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This kinetic intermediate contains three of four native helices that are oriented in a non-native manner so as to minimise exposed hydrophobic surface area at this point in folding. The short 6-residue helix III has been shown to Bless massage and spa Barking unstructured in the intermediate ensemble and does not dock onto the developing hydrophobic core until after the rate-limiting transition state has been Yellow rose modeling institute Fareham. In order to probe further the role of helix III in the folding mechanism of Im7, we Sex clubs Hove county a variant that contains an eight-amino-acid polyalanine-like helix stabilised by a Glu-Arg salt bridge and an Asn-Pro-Gly capping motif, juxtaposed C-terminal to the natural 6-residue helix III. Yellow rose modeling institute Fareham reveal a robust native structure that is not perturbed by the presence of the extended helix III. Mutational analysis performed to probe the folding mechanism of the redeed protein revealed a conserved mechanism involving the canonical three-helical intermediate.

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